pcs2 cyclinb1 gfp (Addgene inc)

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Addgene inc pcs2 cyclinb1 gfp
Pcs2 Cyclinb1 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcs2+cyclinb1+gfp/pCS2%2BCyclinB1-GFP+(Plasmid+%23128426)/pm37289834-283-34-35
Average 93 stars, based on 3 article reviews

pcs2 cyclinb1 gfp - by Bioz Stars, 2026-09

93/100 stars

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Plasmid Preparation:

Article Title: Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes
Article Snippet: .. PCS2-Rab11-mCherry ( ) and PCS2-Rab11S25N-mCherry (see below), PCS2-DCLK-mKO2 (see below), PCS2-Utrophin-GFP , and PCS2-CyclinB1-GFP (Addgene plasmid #128426) expression constructs were used. mRNAs were synthesized using the SP6 mMessage mMachine Kit (ThermoFisher). .. Injections into stage-III oocytes were performed as described in ( ) using glass capillary needles (30-0020, Harvard Apparatus, MA, USA), which were pulled by a needle puller (P-97, Sutter Instrument) and attached to a microinjection system (PV820, World Precision Instruments).

Article Title: Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes.
Article Snippet: The pTol2 vector was coinjected with mRNA encoding the transposase (Invitrogen) into 1 cell-stage wildtype TL embryos. .. Individual positive carriers were selected and out-crossed with wild-type TL fish for stable single-copy genetic integration. mRNA injections PCS2-Rab11-mCherry ([59]; N-terminally tagged) and PCS2-Rab11S25N-mCherry (see below; N-terminally tagged), PCS2-DCLK-mKO2 (see below), PCS2-Utrophin-GFP [60], and PCS2-CyclinB1-GFP (Addgene plasmid #128426) expression constructs were used. mRNAs were synthesized using the SP6 mMessage mMachine Kit (Thermo Fisher). .. Injections into PLOS Biology | https://doi.org/10.1371/journal.pbio.3002146 June 8, 2023 18 / 35 stage III oocytes were performed as described in [53] using glass capillary needles (30–0020, Harvard Apparatus, MA, USA), which were pulled by a needle puller (P-97, Sutter Instrument) and attached to a microinjection system (PV820, World Precision Instruments).

Article Title: Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes
Article Snippet: .. PCS2-Rab11-mCherry ([ ]; N-terminally tagged) and PCS2-Rab11S25N-mCherry (see below; N-terminally tagged), PCS2-DCLK-mKO2 (see below), PCS2-Utrophin-GFP [ ], and PCS2-CyclinB1-GFP (Addgene plasmid #128426) expression constructs were used. mRNAs were synthesized using the SP6 mMessage mMachine Kit (Thermo Fisher). .. Injections into stage III oocytes were performed as described in [ ] using glass capillary needles (30–0020, Harvard Apparatus, MA, USA), which were pulled by a needle puller (P-97, Sutter Instrument) and attached to a microinjection system (PV820, World Precision Instruments).

Expressing:

Article Title: Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes
Article Snippet: .. PCS2-Rab11-mCherry ([ ]; N-terminally tagged) and PCS2-Rab11S25N-mCherry (see below; N-terminally tagged), PCS2-DCLK-mKO2 (see below), PCS2-Utrophin-GFP [ ], and PCS2-CyclinB1-GFP (Addgene plasmid #128426) expression constructs were used. mRNAs were synthesized using the SP6 mMessage mMachine Kit (Thermo Fisher). .. Injections into stage III oocytes were performed as described in [ ] using glass capillary needles (30–0020, Harvard Apparatus, MA, USA), which were pulled by a needle puller (P-97, Sutter Instrument) and attached to a microinjection system (PV820, World Precision Instruments).

Construct:

Article Title: Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes
Article Snippet: .. PCS2-Rab11-mCherry ([ ]; N-terminally tagged) and PCS2-Rab11S25N-mCherry (see below; N-terminally tagged), PCS2-DCLK-mKO2 (see below), PCS2-Utrophin-GFP [ ], and PCS2-CyclinB1-GFP (Addgene plasmid #128426) expression constructs were used. mRNAs were synthesized using the SP6 mMessage mMachine Kit (Thermo Fisher). .. Injections into stage III oocytes were performed as described in [ ] using glass capillary needles (30–0020, Harvard Apparatus, MA, USA), which were pulled by a needle puller (P-97, Sutter Instrument) and attached to a microinjection system (PV820, World Precision Instruments).

Synthesized:

Article Title: Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes
Article Snippet: .. PCS2-Rab11-mCherry ([ ]; N-terminally tagged) and PCS2-Rab11S25N-mCherry (see below; N-terminally tagged), PCS2-DCLK-mKO2 (see below), PCS2-Utrophin-GFP [ ], and PCS2-CyclinB1-GFP (Addgene plasmid #128426) expression constructs were used. mRNAs were synthesized using the SP6 mMessage mMachine Kit (Thermo Fisher). .. Injections into stage III oocytes were performed as described in [ ] using glass capillary needles (30–0020, Harvard Apparatus, MA, USA), which were pulled by a needle puller (P-97, Sutter Instrument) and attached to a microinjection system (PV820, World Precision Instruments).

Review